DNA EXTRACTION

INTRODUCTION:
Desoxyribonucleics acid is a nucleic acid that encodes the genetic instructions udes in the development and functioning of all know living organisms and many viruses.

MATERIALS: 

-1L Efety gogglesrlenmeyer flisk

-100mL beaker
-10mL graduated cylinder
-Small funnel
-Glass stirring rod
-10mL Pipet
-Knife
-Safety goggles
-Cheesecloth
-Kiwi
-Banana
-Pineapple juice
-Distilled water
-90% ethanol ice-cold
-7mL DNA buffer
-50 mL dish soap
-15g NaCl
-900mL tap water

PROCEDURE:

Put the ethanol in the freezer-you will need it really cold later!
Prepare the buffer in a 0'5L beaker:
-450mL of a tap water
-25mL of dish soap
-7g NaCl. Stir the mixture.







1.Peel the kiwi and chop it to small piece. Place the pieces of the kiwi in one 600mL beaker and smash with a fork until it becomes a juice puree.
2.Add 8mL of buffer to the beaker.
3.Mash the kiwi puree carefully for 3 minutes without creating many bubbles.
4.Filter the mixture: put funnel on top of the gradduated cylinder. Place the cheesecloth on top of the funnel.






5.Add beaker contain  carefully on top of the cheesecloth to fill the graduated cylinder. The juice will drain through the cheesecloth but the chucks of kiwi will not pass throught into the graduated cylinder.
6.Add the pineapple juice to the green juice. This step will help us to obtain a purer solution of DNA. Pineapple juice contains an ezyme that breaks down proteins.
7.Tilt the graduated cylinder and pour in an equal amount of ethanol with an automatic pipet. Put the ehanol through the sides of the graduated cylinder very carrefully. You will need about equal volumes of DNA solutions to ethanol.
8.Place the graduated cylinder so that it is eye level. Using the stirring rod, collect DNA at the boundary of ethanol and kiwi juice. Don't stir the kiwi juice, only stir in the above ethanol layer.
9.The DNAprecipitate looks like long . white and thin fibers.
10.Gently remove the stirring rod and examine what the DNA looks like.

(Roger's photo blog)

QUESTIONS:
1.Like a gelatine, colour white.
2.To brake the cells. It is located in the cell wall
3.We add salt to brake the cells and soap to clean the proteins.
4.It is a good methond to catch it bacause the DNA goes to ethanol.