MATERIALS:
-Toothpick
-2 slides
-2 covers slips
-Distilled water
-Methylene blue
-Iodine
-Onion
-Glycerine
OBJECTIVES:
1.Identify the major components of cells.
2.Differetiale between animal and plant cells.
3.Mesure dimensions of the entire cell and the nucleus
PROCEDURE:
Plant cell observation
1.Pour some distilled water into a watch glass
2.Peel off the leaf from half a piece of onion and using forceps, pull out a piece of transperent onion peel (epidermis) from the leaf.
3.Put the epiderms in the watch glass containing distilled water.
4.Take a few drops of iodine solution in a dropper and transfer into another watch glass.
5.Using a brush, transfer the peel into the watch glass containing the dye. Let this remain in the safranin solution for 30 seconds, so that the peel is stained.
6.Take the peel from the iodine solution and place it in the watch glass containing distilled water.
7.Take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide
8.Using the brush, place the peel into the slide containing glycerine.
9.Take a cover slip and place it gently on the peel with the aid of a needle.
10.Remove the extra glycerine using cellulose paper.
11.View it in the microscope.
Please Lola, can you post any image?
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