Saccharides are organic molecules consisting of C, H and O atoms. The empirical formula usually is Cn H2n On. Are divided in two groups:
-Monosaccharides: formed by linear a carbon chain, are the building blocks of oligo and polysaccharides. They have a one functional group: cetone (C=O) or aldehyde (COH).
-Oligosaccharides: small polymer containing between 2 and 10 monosaccharides.
-Polysaccharides: big polymers with more than 10 monosaccharides.
Materials:
-Test tube rack
-10mL Pipet
- 5 spatula
-5 test tubes
-1 dropper
-Distilled water
-Lactose
-Maltose
-Glucose
-Sucrose
-Starch
-Lugol's iodine
-Maltose
-Glucose
-Sucrose
-Starch
-Lugol's iodine
Objectives:
1- Identify
different sugars from its properties.
2- Differentiate
monosaccharides and disaccharides.
3- Understand the
relation between structure and some proporties.
Procedure:
We started off by testing some physical properties of the saccharides we have in the lab, such as flavour, the structure and color.
To test the flavour we tasted a small part of each saccharide, and we asked ourselves wether it was sweet or sour?
And by obvserving a small amount of each saccharide under magnification we tested the crystal.
Lastly we tested the color of each element, was it creamy, white or transparent?
GLUCOSE
|
MALTOSE
|
SUCROSE
|
LACTOSE
|
STARCH
|
||
Flavour
|
sweet
|
sweet
|
sweet
|
no sweet
|
no sweet
|
|
Clystalline
|
crystaline
|
no crystaline
|
crystaline
|
powder
|
crystaline
|
|
Colour
|
white
|
cream
|
white
|
white
|
white
|
|
Solubility
|
soluble
|
insoluble
|
soluble
|
soluble
|
insoluble
|
|
Lugol’s iodine
|
negative
|
negative
|
negative
|
negative
|
positive |
TO TEST SOLUBILITY:
- Clean and dry 5
test tubes and label them: G, M, L, SU, S
-Pour 5mL of water
in each test tube.
-With the help of a
spatula, put a small amount of each saccharide inside the labelled test tube
and test if they are soluble or insoluble.
- Observe is each
saccharide forms a mixture called dissolution or a colloidal suspension.
- Finally, add 2
drops of Lugol's iodine to each test tube and test if the reaction is positive
or negative. Lugol's is a solution of elemental iodine (I) and potassium iodine
(KI) in water that is ise to test a saccharide. The reaction is positive if iodine reacts by turning from yellow to a purple, dark-blueish colour.
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