LEAF PIGMENTS CHROMATOGRAPHY:

OBJECTIVE:
We are going to do a paper chromatography.

MATERIAL:
-Mortal and pestle
-Scissor
-Funnel
-Graduated cylinder
-Beaker (250ml)
-Sand
-Ethanol
-Spinacks
-CaCO3
-Cellulose paper

PROCEDURE:




-Take ten spinacks
-Cut it in small pieces in the mortal (Not take the nerve)
-Put the sand in the mortal with a spatula
-Add CaCo3 in the mortal with a spatula
-Add 50ml the ethanol in the mortal

 -Then you need to grind
-Filter this in a graduated cylinder with cellulose paper and put all in the beaker
-Create a small paper and put inside the beacker
-Create other small paper fold it and put into other beaker

QUESTIONS:
1.Why do we add sand?
For brake the cell and cloroplast.

2.Why do we add calcium carbonate?
For avoid the pigmnets degradesion.

3.Which is the colour of every pigmnets?
Clorofila green, xantofil yellow and carotenoide orange.

4.What adaptative purpose do differents colored pigmnets serve for a plant?
Because the light have diferents wave lenghts and the pigmnets take this diferents waves lenghts.

5.Why do they separate on the cellulose paper?
Because not all the pigmnets have the same solubility.

CHROMOPLAST AND AMILOPLAST:

INTRODUCTION:
The chromoplast store cloroplast. And the amiloplast is chain of milo.

PROCEDURE WITH TOMATO:
-Take a squash of tomato
-Put the squash in a cover slip
-With a 2cm paper tight the squash
-Dry the squash of tomato
-Observe the cell and try to see the pigments














PROCEDURE WITH POTATO:
-Cut the potato and take a white liquid
-Put this in a cover slip
-Dry the white liquid
-Put lugol and wait 3minutes
-Throw the lugol with distilled water
-Observe the sample

OBSERVATIONS:
The cells are transparents But the pigmnets create the colour red of the potato. This pigmnets are inside the cloroplast.